Three-dimensional printing is a new raised technology, which can be used to control the shape of the scaffold and its inside structure. This technology has great potential in building tissue engineering scaffold, which can fulfill the requirement of personalized medicine. In recent years, Bio-3D printing technology has attracted more and more researchers’ attention, which breaks through the limitation of low spatial resolution of traditional tissue engineering technology, and can precisely control the distribution of cells in scaffold material, cell survival of the microenvironment, to provide a true three-dimensional balanced growth environment, making complex tissue and organ repair and in vitro reconstruction. This review introduces the three-dimensional bioprinting technology and the construction of bioinks, as well as the application in tissue and organ regeneration.