Peripheral nerve injury and amputation have plagued neurosurgeons and patients. Autologous nerve grafts has been known as the “golden rule” to repair severe lesions but is limited by the length and potentially losing functions originally from the donor nerves. Meanwhile, tissue-electrode interface was proposed to overcome the limitation of the traditional neural interface, but long autologous nerves with original functions are preferred. So finding out a substitute material to replace the autologous nerves as donor nerves becomes critical. Dorsal root ganglia (DRG) proved an ideal original material. In this report, we described a method to isolate the DRG explants from the spinal cord of SD rat, and coated the Aclar films with poly-D-Lysine (PDL) and poly-L-Lysine (PLL) respectively. Then the strategy of culturing the DRG explants on the coated Aclar films was introduced. An experiment was designed to investigate the growth of the DRG explants, specifically the DRG axons cultured on the two separately coated -Aclar films. The results of the experiment show that PDL has an advantage in coating the Aclar film for culturing the DRG explants for nerve tissue growth.