An electrophysiological glasses based on independent chipset was proposed. It consisted of signal acquisition module, signal preprocessing module, microcontroller module and signal analysis module. Due to the high input impedance, active electrode, instead of conductive paste, was used as signal acquisition module, which could improve system availability. The signal preprocessing module took advantage of electrophysiological analog front-end to filter and amplify electrophysiological signal, thus removed interference and enhanced the signal to noise ratio. Cortex-M0 processor of stmicroelectronics was adopted as the microcontroller module for analog-to-digital conversion, analog front-end setup, and controlling of bluetooth module transceiver. The signal analysis module could realize digital filtering, feature extraction, and heart rate monitoring for electrophysiological signal. Based on this, experiments and signal analysis about neck electrocardiogram and brain electrical interaction were carried out.