Optogenetics technology has the advantage of regulating the activity of specific cell population in milliseconds level, which provides a novel bioengineering tools for understanding the mechanism of the neural circuits and the pathogenesis of some neurological diseases and interventions treatment. Based on the characteristics of optogenetics, we developed a set of LED light stimulation system for optogenetics modified in vitro cell culture or brain slice. This device can remotely control stimulation parameters of LED arrays such as time, frequency, duty cycle, and simultaneously detect physicochemical properties of cultured cells.