The performance of automotive under specific drive cycles can provide a reference for the development and improvement of vehicle systems. At present, there is no representative typical driving cycle in Shenzhen. In this paper, the development of M347 bus route driving cycle in Shenzhen was reported, combining principal component analysis and clustering analysis methods, and the rationality and the validity of the driving cycle were tested and verified. The original micro-trips are divided into two series by the clustering analysis method, which may explain traffic jam and smooth traffic driving conditions of M347 bus route. The M347 route multiple driving cycle constructed at last can reflect the real-world road conditions.